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Merck
CN
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文件

574777

Sigma-Aldrich

兔抗突触素I多克隆抗体

lyophilized, Calbiochem®

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UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

形式

lyophilized

不包含

preservative

种属反应性

human, rat, mouse, bovine

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

同位素/亚型

IgG

运输

ambient

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

mouse ... Syn1(20964)

一般描述

免疫亲和纯化的兔多克隆抗体。识别突触I蛋白。
识别大鼠大脑中的突触I蛋白。
这种抗突触蛋白I兔pAb经验证可用于 ELISA、免疫印迹、免疫细胞化学、免疫沉淀,以检测突触蛋白I。

免疫原

纯化的牛脑突触素I

应用

ELISA(1:2500-1:10000)

冰冻切片(见应用参考文献)

免疫印迹(1:200)

免疫细胞化学(1:500-1:2500)

免疫沉淀(1 µg/200 µg裂解物)

警告

毒性:标准处理(A)

外形

从5 mM碳酸氢铵中冻干。

重悬

用50µl PBS复溶。复溶后,等分并冷冻保存(-20°C)。避免溶液反复冻融。

分析说明

大鼠脑
阳性对照

其他说明

Stone, L.M., et al. 1994.J. Neurosci.14, 301.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
该抗体对突触蛋白I具有特异性,可用于估计突触密度和突触发生。在1:200稀释的免疫印迹中可以看到突触蛋白I双峰的强而特异的免疫标记。这种免疫标记可以通过抗体与突触蛋白I的预吸附来阻止。与测定条件相关的变量将决定适当的工作稀释度。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1


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