推荐产品
产品名称
Ru360, Ru360, is a cell-permeable oxygen-bridged dinuclear ruthenium amine complex. Binds to mitochondria with high affinity (Kd = 340 pM) and blocks Ca2+ uptake into mitochondria in vitro (IC₅₀ = 184 pM).
质量水平
方案
≥97%
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
protect from light
颜色
green
溶解性
deoxygenated water: 0.5 mg/mL
运输
ambient
储存温度
−20°C
一般描述
一种可渗透性氧桥联双核钌胺络合物，已显示出与线粒体结合的高亲和力 (Kd = 0.34 nM)并可特异性阻断Ca2+在体外（IC50 = 184 pM）线粒体中和在原位完整心肌细胞中的摄取（在~10 µM的Ru360中孵育30分钟后完全阻断。即使在微摩尔水平下也不会影响参与心肌收缩的其他细胞Ca2+转运过程。
一种细胞渗透性氧桥联双核钌胺络合物，已显示出与线粒体结合的高亲和力 (Kd = 340 pM)。特异性阻断Ca2+在体外（IC50 = 184 pM）线粒体中和在原位完整心肌细胞中的摄取（在~10 µM的Ru360中孵育30分钟后完全阻断）。即使在微摩尔水平下也不会影响参与心肌收缩的其他细胞Ca2+转运过程。
注意：1组 = 10 x 100 µg.
生化/生理作用
产物不与ATP竞争。
可逆：否
细胞可渗透性：具有
靶标IC50：184 pM 阻断Ca2+在体外摄取至线粒体中；以及在原位完整心肌细胞中；结合线粒体的Kd = 340 pM
首要靶标
Ca2+在体外摄取至线粒体中
Ca2+在体外摄取至线粒体中
包装
用惰性气体包装
警告
毒性：标准处理（A）
重悬
溶液中不稳定；仅在使用前进行重悬。
其他说明
Sanchez, J.A., et al. 2001.J. Physiol. 536, 387.
Bassani, R.A., et al. 1998.Cell Calcium23, 433.
Matlib, M.A., et al. 1998.J. Biol. Chem.273, 10223.
Zhou, Z., et al. 1998.J. Physiol.507 (pt 2), 379.
Emerson, J., et al. 1993.J. Am. Chem. Soc.115, 11799.
Ying, W.-L., et al. 1991.Biochemistry30, 4949.
Bassani, R.A., et al. 1998.Cell Calcium23, 433.
Matlib, M.A., et al. 1998.J. Biol. Chem.273, 10223.
Zhou, Z., et al. 1998.J. Physiol.507 (pt 2), 379.
Emerson, J., et al. 1993.J. Am. Chem. Soc.115, 11799.
Ying, W.-L., et al. 1991.Biochemistry30, 4949.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Rachel L Doser et al.
eLife, 13 (2024-03-14)
Our understanding of mitochondrial signaling in the nervous system has been limited by the technical challenge of analyzing mitochondrial function in vivo. In the transparent genetic model Caenorhabditis elegans, we were able to manipulate and measure mitochondrial reactive oxygen species
Madison X Rodriguez et al.
STAR protocols, 2(4), 100979-100979 (2021-12-09)
The mitochondrial calcium uniporter, which mediates mitochondrial Ca2+ uptake, regulates key cellular functions, including intracellular Ca2+ signaling, cell-fate determination, and mitochondrial bioenergetics. Here, we describe two complementary strategies to quantify the uniporter's transport activity. First, we detail a mitochondrial Ca2+
Michael J Bround et al.
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Mitochondrial Ca2+ overload can mediate mitochondria-dependent cell death, a major contributor to several human diseases. Indeed, Duchenne muscular dystrophy (MD) is driven by dysfunctional Ca2+ influx across the sarcolemma that causes mitochondrial Ca2+ overload, organelle rupture, and muscle necrosis. The
Lianne Pope et al.
Cell chemical biology, 27(5), 511-524 (2020-02-16)
The trinuclear ruthenium amine ruthenium red (RuR) inhibits diverse ion channels, including K2P potassium channels, TRPs, the calcium uniporter, CALHMs, ryanodine receptors, and Piezos. Despite this extraordinary array, there is limited information for how RuR engages targets. Here, using X-ray
MCU Upregulation Overactivates Mitophagy by Promoting VDAC1 Dimerization and Ubiquitination in the Hepatotoxicity of Cadmium.
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Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2203869-e2203869 (2023)
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