推荐产品
质量水平
形式
liquid
比活
≥20,000 units/mL
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
异质活性
Endonuclease and RNase, none detected
运输
wet ice
储存温度
−20°C
一般描述
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
生化/生理作用
Cell permeable: no
Primary Target
inactivates RNase
inactivates RNase
Product does not compete with ATP.
Reversible: no
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
外形
In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.
重悬
Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.
其他说明
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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