跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

539779

Millipore

ProteoExtract® Complete Mammalian Proteome Extraction Kit

登录查看公司和协议定价
所有图片(1)
别名:
C-PEK Mammalian Kit
UNSPSC代码:
41106510
NACRES:
NA.32

用途

20 extractions

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

输入

sample type: mammalian tissue(s)
sample type: mammalian cultured cells

运输

ambient

储存温度

2-8°C

一般描述

A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue.

包装

1 kit in Glass bottle

组分

Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS

原理

ProteoExtract® Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mammalian tissue culture cells or mammalian tissue.

储存及稳定性

  • Wash Buffer
The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C.
  • Resuspension Buffer
The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!
  • Extraction Reagent
The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!
  • Reducing Agent
The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use.

Avoid repeated freezing and thawing!
  • Benzonase®
Benzonase® should be stored at 4°C.

其他说明

Yuan, X., et al. 2002. Electrophoresis 23, 1185. Allen, L. 2000. Functional Genomics Nature 405 819. Herbert, B. 1999. Electrophoresis 20, 660.

法律信息

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2

WGK

WGK 3

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门