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539480

Sigma-Aldrich

蛋白酶 K 来源于林伯氏白色念球菌

别名:

肽链内切酶

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About This Item

CAS号:
39450-01-6
EC 号:
3.4.21.14 (BRENDA, IUBMB)
EC 号:
3.4.21.64 (BRENDA, IUBMB)
MDL编号:
MFCD00132129
UNSPSC代码:
12352202
NACRES:
NA.21

表单

lyophilized solid

质量水平

400

比活

≥30 mAnson units/mg dry wt
≥40 mAnson units/mg protein

分子量

28.93 kDa

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

颜色

white

溶解性

50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0: soluble

异质活性

DNase, none detected (nicking activity with pBR322, incubation for 6 h at 37°C)
RNase, none detected (ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C)

运输

ambient

储存温度

2-8°C

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一般描述

丝氨酸蛋白酶，对多种变性和天然的高分子量蛋白质具有很强的蛋白水解活性。它可使内源性核酸酶迅速蛋白水解失活，因此可用于分离mRNA和高分子量DNA。优先裂解N-取代疏水性、脂肪族和芳香族氨基酸羰基旁的键。可被DFP、Hg2+和PMSF抑制。不会被金属螯合剂、巯基试剂、TLCK或TPCK灭活。

应用

在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

生化/生理作用

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

警告

毒性：刺激性（B）

单位定义

一个mAnson单位定义为在37°C、pH 7.5条件下每分钟释放1.0 µM Folin阳性氨基酸（以酪氨酸计）的酶的量。

外形

由乙酸钙冻干

重悬

复溶后，储存在冰箱中（4°C）。用50 mM Tris-HCl、2 mM乙酸钙制备的pH 8.0的储备溶液在4°C下可稳定保存1年。

其他说明

Watazu, Y., et al. 1993.J. Lab. Clin. Anal.7, 81.
Lebherz, H.G., et al. 1986.生物化学。J.233, 51.
Ebeling, W., et al. 1974.Eur. J. Biochem.47, 91.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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International journal of molecular sciences, 23(23) (2022-12-12)
Retinitis pigmentosa (RP) is a group of inherited retinal dystrophies that typically results in photoreceptor cell death and vision loss. Here, we explored the effect of early growth response-1 (EGR1) expression on photoreceptor cell death in Pde6brd1 (rd1) mice and
Richard P Tucker et al.
Methods in molecular biology (Clifton, N.J.), 2047, 421-437 (2019-09-26)
One of the first steps in studies of gene function is the spatiotemporal analysis of patterns of gene expression. Indirect immunohistochemistry is a method that allows the detection of a protein of interest by incubating a histological section with an
Markus Siegert et al.
Drug testing and analysis, 11(5), 659-668 (2018-11-24)
Exposure to the vesicant sulfur mustard (SM) may lead to erythema and blistering. Toxicity of SM is hypothesized due to the alkylation of DNA bases and nucleophilic amino acid side chains in proteins (adducts) by forming the hydroxyethylthioethyl (HETE) moiety.
Kongyang Zhu et al.
iScience, 25(12), 105636-105636 (2022-12-31)
A high point of Tibetan Plateau (TP) civilization, the expansive Tubo Empire (618-842 AD) wielded great influence across ancient western China. However, whether the Tubo expansion was cultural or demic remains unclear due to sparse ancient DNA sampling. Here, we
Markus Siegert et al.
Drug testing and analysis, 10(2), 243-253 (2017-09-08)
The vesicant sulfur mustard (SM) is a banned chemical warfare agent. Although, SM has been used in combat since WWI, there is no causal therapy currently available. Accordingly, development and investigation of antidotes and scavengers targeting SM are of high
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