形式
liquid
质量水平
包含
≤0.1% sodium azide as preservative
制造商/商品名称
Calbiochem®
储存条件
do not freeze
运输
wet ice
储存温度
2-8°C
一般描述
Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
Protein G, Alkaline Phosphatase Conjugate is obtained by conjugation of protein G, a cell-surface protein of Streptococcus species to alkaline phosphatase. This conjugate is used as a secondary agent in immunoassay methods to detect polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a, and IgG2b.
应用
Protein G, Alkaline Phosphatase Conjugate has been used in:
- direct enzyme-linked immunosorbent assay (ELISA) (1:1000)(1:250)
- indirect ELISA (1:1000)
- immunoblot (1:5000)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
分析说明
Single band at 33.8 kDa by SDS-PAGE
其他说明
p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
S Sun et al.
Journal of immunological methods, 152(1), 43-48 (1992-07-31)
The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This
A O Olarinmoye et al.
Epidemiology and infection, 145(10), 2030-2037 (2017-05-10)
The hamadryas baboon (Papio hamadryas hamadryas) is the only indigenous species of non-human primates (NHP) found in the Kingdom of Saudi Arabia (KSA). There are no peer-reviewed publications on viral infections of the baboons of KSA. Apart from camels, other
B Nilson et al.
Journal of immunoassay, 9(2), 207-225 (1988-01-01)
Protein G, an IgG-binding protein, purified from the surface of group G streptococci, was coupled to alkaline phosphatase. The conjugate was used for detection of polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a and IgG2b in an enzyme-linked
Lisa D MacDonald et al.
Human vaccines & immunotherapeutics, 14(1), 59-66 (2017-09-22)
Peptide antigens are combined with an adjuvant in order to increase immunogenicity in vivo. The immunogenicity and safety of a RSV vaccine formulated in a novel oil-based platform, DepoVax™ (DPX), was compared to an alum formulation. A peptide B cell
Anja Reckendorf et al.
International journal for parasitology. Parasites and wildlife, 14, 53-61 (2021-01-26)
Pseudaliid lungworm (Metastrongyloidea) infections and associated secondary bacterial infections may severely affect the health status of harbour porpoises (Phocoena phocoena) in German waters. The presented retrospective analysis including data from 259 harbour porpoises stranded between 2006 and 2018 on the
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