跳转至内容
Merck
CN
所有图片(1)

文件

539137

Sigma-Aldrich

蛋白酶抑制剂混合物套装V,无EDTA

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases.

登录查看公司和协议定价

别名:
EDTA free Protease inhibitor
UNSPSC代码:
12352200
NACRES:
NA.54

质量水平

形式

lyophilized

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
desiccated (hygroscopic)

运输

wet ice

储存温度

−20°C

一般描述

四种蛋白酶抑制剂的混合物,用于抑制丝氨酸、半胱氨酸,但不用于抑制金属蛋白酶。推荐用于哺乳动物细胞裂解液和组织提取物品。以单瓶或10瓶一组的形式提供。复溶时,每个小瓶含有100X的贮备溶液。当稀释至1X时,请混合以下成分:
四种蛋白酶抑制剂的混合物,用于抑制丝氨酸、半胱氨酸,但不用于抑制金属蛋白酶。用1 ml H2O复溶每个小瓶,以获得100X溶液。1X贮备溶液,含有500 µM AEBSF、HCl(目录号101500)、150 nM抑肽酶(目录号616370)、1 µM E-64(目录号324890)和1 µM亮抑蛋白酶肽(目录号108975)。随附数据表。

特异性

抑制丝氨酸蛋白酶、半胱氨酸蛋白酶。切勿抑制金属蛋白酶。

生化/生理作用

主靶
丝氨酸和半胱氨酸蛋白酶,但不用于抑制金属蛋白酶。
产物不与ATP竞争。
可逆:否
细胞可渗透性:否

警告

毒性:标准处理(A)

重悬

复溶后,等分并冷冻保存(-20°C)。贮备溶液在-20°C下可稳定保存至多6个月。
用1 ml H₂O复溶每个小瓶,以获得100X溶液。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Corr. 1A

WGK

WGK 3


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Mario Lebendiker et al.
Methods in molecular biology (Clifton, N.J.), 1485, 257-273 (2016-10-13)
Maltose-Binding Protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows the use of a simple capture affinity step on Amylose-Agarose or Dextrin-Sepharose columns, resulting in a protein that
Kirby Martinez-Fonts et al.
Nature communications, 11(1), 477-477 (2020-01-26)
Proteins are targeted to the proteasome by the attachment of ubiquitin chains, which are markedly varied in structure. Three proteasome subunits-Rpn10, Rpn13, and Rpn1-can recognize ubiquitin chains. Here we report that proteins with single chains of K48-linked ubiquitin are targeted
Emery T Usher et al.
The Journal of biological chemistry, 296, 100693-100693 (2021-04-25)
Speckle-type POZ protein (SPOP) is a ubiquitin ligase adaptor that binds substrate proteins and facilitates their proteasomal degradation. Most SPOP substrates present multiple SPOP-binding (SB) motifs and undergo liquid-liquid phase separation with SPOP. Pancreatic and duodenal homeobox 1 (Pdx1), an
Jin Wen et al.
Bioorganic & medicinal chemistry, 28(20), 115711-115711 (2020-10-18)
Cyclic peptides are capable of binding to challenging targets (e.g., proteins involved in protein-protein interactions) with high affinity and specificity, but generally cannot gain access to intracellular targets because of poor membrane permeability. In this work, we discovered a conformationally
Chinyere Agbaegbu Iweka et al.
Journal of neurochemistry, 157(3), 494-507 (2020-12-16)
Phospholipid Phosphatase-Related Protein Type 1 (PLPPR1) is a member of a family of lipid phosphatase related proteins, integral membrane proteins characterized by six transmembrane domains. This family of proteins is enriched in the brain and recent data indicate potential pleiotropic

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门