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Merck
CN
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主要文件

53702

Millipore

Pronase®蛋白酶,灰色链霉菌

别名:

Pronase® powder

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.54

表单

lyophilized

质量水平

比活

≥45,000 proteolytic units/g dry wt

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
desiccated (hygroscopic)

溶解性

water: 10 mg/mL

运输

ambient

储存温度

2-8°C

一般描述

一种非特异性蛋白酶将粘蛋白液化并消化蛋白质以释放氨基酸。可用于分离活的软骨细胞。在中性pH下最具活性,但可在较宽的pH和温度范围内稳定。最适pH为7.5。注意:1 KU = 1000单位。
一种非特异性蛋白酶将粘蛋白液化并消化蛋白质以释放氨基酸。可用于分离活的软骨细胞。在中性pH下最具活性,但可在较宽的pH和温度范围内稳定。注意:1 KU = 1000单位。
注意:1 KU = 1000单位。

警告

毒性:有害(C)

单位定义

一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。

外形

从含有乙酸钙作为稳定剂的溶液进行冻干。

重悬

在重悬后存放于冰箱(4°C)。储备液可在4°C稳定24小时。

其他说明

Narahashi, Y., et al. 1968.J. Biochem.64, 427.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 2


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T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus.
R F Keeler et al.
Journal of general microbiology, 43(3), 439-454 (1966-06-01)
Nicole M Templeman et al.
Developmental cell, 54(1), 92-105 (2020-06-17)
Evolutionarily conserved signaling pathways are crucial for adjusting growth, reproduction, and cell maintenance in response to altered environmental conditions or energy balance. However, we have an incomplete understanding of the signaling networks and mechanistic changes that coordinate physiological changes across
Jun Sunayama et al.
Stem cells (Dayton, Ohio), 28(11), 1930-1939 (2010-09-22)
The molecular signaling pathways orchestrating the biology of cancer stem-like cells (CSLCs), including glioblastoma, remain to be elucidated. We investigated in this study the role of the MEK/extracellular signal-regulated kinase (ERK) pathway in the control of self-renewal and tumorigenicity of
Yifei Jiang et al.
Anesthesiology, 125(6), 1159-1170 (2016-09-23)
Exposure to isoflurane increases apoptosis among postnatally generated hippocampal dentate granule cells. These neurons play important roles in cognition and behavior, so their permanent loss could explain deficits after surgical procedures. To determine whether developmental anesthesia exposure leads to persistent

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