535142
Protease Inhibitor Cocktail I
Animal-Free, lyophilized, for the inhibition of serine, esterases, cysteine, trypsin-like and metalloproteases. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
别名:
Animal-Free Protease Inhibitor Mix, Protease inhibitor
登录查看公司和协议定价
所有图片(1)
About This Item
UNSPSC代码:
12352200
NACRES:
NA.77
表单:
lyophilized
储存温度:
−20°C
推荐产品
产品名称
Protease Inhibitor Cocktail Set I, Animal-Free, The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
表单
lyophilized
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
运输
wet ice
储存温度
−20°C
一般描述
A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H2O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.
应用
Protease Inhibitor Cocktail Set I, Animal-Free has been used:
- as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines
- in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting
- as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell
生化/生理作用
Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.
警告
Toxicity: Standard Handling (A)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
靶器官
Respiratory Tract
储存分类代码
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Rongxuan Zhu et al.
Frontiers in oncology, 12, 848483-848483 (2022-04-26)
Rapid tumor growth inevitably results in energy stress, including deficiency of glutamine, a critical amino acid for tumor cell proliferation. However, whether glutamine deficiency allows tumor cells to use lipid droplets as an energy resource and the mechanism underlying this
Waqas Azeem et al.
Frontiers in immunology, 11, 438-438 (2020-04-16)
The transcription factor β-catenin is able to induce tolerogenic/anti-inflammatory features in different types of dendritic cells (DCs). Monocyte-derived dendritic cells (moDCs) have been widely used in dendritic cell-based cancer therapy, but so far with limited clinical efficacy. We wanted to
Detection of HBV C Protein Phosphorylation in the Cell
Jung J and Kim K
Bio-protocol, 5(15), e1551-e1551 (2015)
Ca2+/Calmodulin-dependent protein kinase II inhibits hepatitis B virus replication from cccDNA via AMPK activation and AKT/mTOR suppression
Kim J, et al.
Microorganisms, 10(3), 498-498 (2022)
Yan Yan et al.
Cancer communications (London, England), 40(12), 694-710 (2020-10-06)
Dopamine and dopamine receptor D1 (DRD1), a member of the dopamine receptor family, have been indicated to play important roles in cancer progression, but dopamine secretion in hepatocellular carcinoma (HCC) and the effects of DRD1 on HCC remain unclear. This
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门