529625
Polyphosphoinositide-Binding Peptide, PBP10
A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin.
别名:
Polyphosphoinositide-Binding Peptide, PBP10, RhoB-GS 160-169, Rhodamine B-QRLFQVKGRR-OH, RhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH
登录查看公司和协议定价
所有图片(1)
About This Item
推荐产品
质量水平
方案
≥95% (HPLC)
表单
lyophilized solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
protect from light
颜色
pink-red
溶解性
DMSO: 2 mg/mL
荧光
λex 565 nm
λem 590 nm
运输
ambient
储存温度
−20°C
一般描述
A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.
A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.
生化/生理作用
Cell permeable: yes
Primary Target
Specifically binds to polyphosphoinositide (PPI)
Specifically binds to polyphosphoinositide (PPI)
Product does not compete with ATP.
Reversible: yes
包装
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
序列
Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Bucki, R., et al. 2001. Biochemistry40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门