PP2

An Introduction to Inhibitors and Their Biological Applications

QCM™ Gelatin Invadopodia Assays provide optimized materials and protocols to enable reproducible analysis of invadopodia in invasive tumor cells (Catalog No. ECM670 for green fluorescence, Catalogue No. ECM671 for red fluorescence). Reagents are provided for coating glass culture surfaces with fluorescent matrix and for colocalizing the actin cytoskeleton and nuclei with invadopodial degradation sites. This assay may also be used for assessing the activity of inhibitors and promoters of invadopodia formation and function. Furthermore, different cell types and individual cells in heterogeneous populations may be analyzed for invasive potential. Finally, the assay kits provide troubleshooting suggestions, recommendations for coating on multiple substrate formats, and example studies in several assay systems (e.g., various cell types, time-course studies, degradation modulation).

Human Kinome Poster: The InhibitorSelect™ Protein Kinase Inhibitor Libraries provide broad coverage of the human kinome as shown here. The depicted human kinome dendrogram of 518 kinases are classified into five broad groups, 90 families, and 145 subfamilies. Inhibitor coverage was assigned based upon published data related to potency (IC50, EC50, Kd, etc.) for individual kinases harvested from the literature. Colored dots denote which library contains an inhibitor with demonstrated potent activity against the designated kinase and do not necessarily reflect known specificity of the inhibitor. Coverage of lipid and atypical kinases are depicted as a separate dendrogram. As shown, Calbiochem® Protein Kinase Inhibitor Libraries cover all major kinase families including TK, CMGC, CAMK, AGC, CK1, STE, TKL, as well as Lipid or Atypical kinase families.