product name
磷酸酶抑制剂混合物套装III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
形式
liquid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
运输
wet ice
储存温度
−20°C
相关类别
一般描述
磷酸化和去磷酸化可调节细胞分裂、增殖和凋亡等细胞活动的信号转导过程。蛋白激酶在目标蛋白上的特定氨基酸残基（丝氨酸、苏氨酸或酪氨酸）上添加磷酸基团，改变它们的构象和与配体的相互作用。蛋白质磷酸酶去除磷酸基团，使蛋白质恢复到去磷酸化状态。当从细胞和组织裂解物中提取磷酸化蛋白时，必须保持其磷酸化状态。磷酸酶抑制剂混合物组III是四种磷酸酶抑制剂的混合物，用于广谱抑制丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶。可以1 ml小瓶或一组5个1 ml小瓶的形式提供。
应用
磷酸酶抑制剂混合物套装III已用于：
- 作为裂解缓冲液的补充，制备全睾丸蛋白裂解液，进行蛋白质印迹分析
- 强化冷藏裂解放射免疫沉淀（RIPA）缓冲液，均质化幼虫后肠，以进行蛋白质印迹分析
- 作为细胞裂解缓冲液的成分，提取总蛋白用于蛋白质印迹分析
生化/生理作用
主要靶标
丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶
丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶
产物不与ATP竞争。
可逆：否
细胞渗透性：否
特点和优势
- 方便的即用型配方
- 高品质的产品，具有一致和可重复的性能
- 灵活选择一系列针对不同类别磷酸酶的混合物
组分
每瓶含有1 ml水溶液，含有以下成分：
- 50 mM氟化钠
- 10 mMß-甘油三酯（目录号35675）
- 10 mM十水焦磷酸钠
- 1 mM正钒酸钠
警告
毒性：有毒（F）
外形
在H₂O中。
重悬
为了长期储存，分装冻存（-20°C）。临用前按1：5稀释。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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