推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
C494, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, hamster
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG2a
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein.
Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein. Does not cross-react with the MDR3 P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C494) is validated for use in Paraffin Sections, FC, Frozen Sections, Immunoblotting, ICC, IP for the detection of P-Glycoprotein.
免疫原
Epitope: gene-specific internal cellular epitope present only on the MDR1 isoform of P-glycoprotein
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a human leukemic cell line
应用
Paraffin Sections (5-10 µg/ml, see comments)
Flow Cytometry (see comments)
Frozen Sections (5-10 µg/ml, see comments)
Immunoblotting (see comments)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Flow Cytometry (see comments)
Frozen Sections (5-10 µg/ml, see comments)
Immunoblotting (see comments)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In PBS, 1% BSA.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Cell lines or frozen tissue, such as human liver or colon.
Cell lines or frozen tissue, such as human liver or colon.
其他说明
Does not cross-react with MDR3 P-glycoprotein. Also reported to work for flow cytometry, immunoblotting, and immunoprecipitation. For immunocytochemistry, frozen or paraffin-embedded material may be used. Permeabilization of the cell membrane is required to expose the epitope recognized by this antibody. Variables associated with assay conditions will dictate the proper working dilutions.
Rao, V.V., et al. 1994. Cancer Res.54, 1536.
Toth, K., et al. 1992. Am. J. Pathol.140, 1009.
Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 152.
Toth, K., et al. 1992. Am. J. Pathol.140, 1009.
Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 152.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Regino Mercado-Lubo et al.
Nature communications, 7, 12225-12225 (2016-07-28)
Salmonella enterica serotype Typhimurium is a food-borne pathogen that also selectively grows in tumours and functionally decreases P-glycoprotein (P-gp), a multidrug resistance transporter. Here we report that the Salmonella type III secretion effector, SipA, is responsible for P-gp modulation through
Amélie Petitprez et al.
International journal of oncology, 42(5), 1644-1653 (2013-04-03)
Irinotecan is a major anticancer agent specifically targeting DNA topoisomerase I. Its cytotoxicity is mediated via a two-step process involving accumulation of reversible DNA‑topoisomerase I complexes associated with transient DNA single-strand breaks which subsequently are converted into permanent DNA double-strand
Golam Kibria et al.
Biological & pharmaceutical bulletin, 37(12), 1926-1935 (2014-12-03)
Multi-drug resistance (MDR) of cancers to chemotherapy including doxorubicin (DOX) is mediated by several factors. To design an effective therapy for the treatment of chemotherapy-resistant cancers, it is essential to explore the elements responsible for mediating MDR. However, exploring these
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门