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Merck
CN
所有图片(1)

文件

5.08940

Millipore

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested

别名:

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested, G418, G 418

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About This Item

经验公式(希尔记法):
C20H40N4O10 · 2H2SO4
分子量:
692.71
MDL编号:
UNSPSC代码:
12352200

检测方案

≥98% (TLC)

质量水平

形式

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

储存温度

−20°C

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

一般描述

Recommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

包装

Packaged under inert gas

警告

Toxicity: Harmful (C)

外形

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.

重悬

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

其他说明

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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