所有图片(1)
别名:
DAPI Stain, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
经验公式（希尔记法）:
C16H15N5 · 2HCl
推荐产品
检测方案
≥97% (HPLC)
质量水平
形式
liquid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
荧光
λex ~359 nm
λem ~461 nm
储存温度
−20°C
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
一般描述
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.
应用
- Determination of Ploidy Levels and Nuclear DNA Content in Cryptococcus neoformans by Flow Cytometry: Drawbacks with Variability: This research utilizes DAPI for quantifying nuclear DNA content and determining ploidy levels in Cryptococcus neoformans, providing critical insights into fungal genetics and potential challenges in reproducibility (Chang et al., 2024).
- Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe: Highlights the development of a novel two-photon fluorescence probe for nucleic acid imaging, employing DAPI as a reference for comparing fluorescence characteristics, enhancing molecular imaging techniques (Sun et al., 2024).
- A FACS-based Novel Isolation Technique Identifies Heterogeneous CTCs in Oral Squamous Cell Carcinoma: Applies DAPI for the enumeration and characterization of circulating tumor cells (CTCs), showcasing its importance in cancer diagnostics and the potential for personalized medicine approaches (Chauhan et al., 2024).
生化/生理作用
Cell permeable: yes
Primary Target
DNA
DNA
Reversible: yes
包装
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
外形
A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
重悬
Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
其他说明
Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.
Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.
Russels, W.C., et al. 1975. Nature253, 461.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.
Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.
Russels, W.C., et al. 1975. Nature253, 461.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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