推荐产品
检测方案
≥90% (HPLC)
质量水平
形式
lyophilized solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated
溶解性
water: 2 mg/mL
运输
wet ice
储存温度
−20°C
InChI
1S/C81H156N10O13S/c1-4-7-10-13-16-19-22-25-28-31-34-37-40-55-73(93)86-72(65-105-64-66(104-75(95)57-42-39-36-33-30-27-24-21-18-15-12-9-6-3)63-103-74(94)56-41-38-35-32-29-26-23-20-17-14-11-8-5-2)80(100)91-71(62-92)79(99)89-68(52-44-48-59-83)77(97)87-67(51-43-47-58-82)76(96)88-69(53-45-49-60-84)78(98)90-70(81(101)102)54-46-50-61-85/h66-72,92H,4-65,82-85H2,1-3H3,(H,86,93)(H,87,97)(H,88,96)(H,89,99)(H,90,98)(H,91,100)(H,101,102)/t66?,67-,68-,69-,70-,71-,72-/m0/s1
InChI key
OEDPHAKKZGDBEV-GFPBKZJXSA-N
一般描述
Pam3CSK4 is a selective toll-like receptor 1/2 (TLR1/2) agonist used in the investigation of TLR1/2 function. It is a synthetic lipopeptide for TLR1/2 and mimics the acylated amino terminus of bacterial lipopeptides. Pam3CSK4, with its three lipid chains, facilitates receptor heterodimerization. The two ester-bound lipid chains are inserted into a pocket in TLR2, while the amide-bound lipid chain is inserted into a hydrophobic channel in TLR1. In the context of human uveitis, endophthalmitis, and experimental uveitis models, Pam3CSK4 triggers the expression and secretion of various cytokines and chemokines in intraocular fluids and/or tissues. A cell-permeable, water-soluble synthetic cationic lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein that potently activates monocytes and macrophages. Induces the release of IL-1, IL-6, TNF-α, superoxide, and NO. Promotes the translocation of NF-κB, enhances tyrosine protein phosphorylation and activation of ERK1/2 and MEK1/2.
应用
Pam₃Cys-Ser-(Lys)₄, Hydrochloride has been used to stimulate MODE-K cells to access its effects on epithelial neuropilin-1 (NRP1) protein expression. It has been used as a toll-like receptor 2 (TLR2) ligand to study TLR2 functionalities.
警告
毒性：标准处理（A）
重悬
复溶后，等分并冷冻保存（-20°C）。避免溶液反复冻融。
其他说明
Barrenschee, M., et al. 2010.PLoS One.5, e13889.
Zahringer, U., et al. 2008.Immunology.213, 205.
Toshchakov, V., et al. 2002.Nat. Immunol.3, 392.
Muller, M.R., et al. 2001.Immunology103, 49.
Vergne, I., and Cezanne, L. 1999.Eur. J. Biochem.264, 369.
Diaz-Guerra, M.J., et al. 1996.J. Biol. Chem.271, 30114.
Offermanns, S., et al. 1992.Biochem.J.282, 551.
Seifert, R., et al. 1990.Biochem.J.267, 795.
Hoffmann, P., et al. 1989.Biol. Chem. Hoppe.Seyler.370, 575.
Zahringer, U., et al. 2008.Immunology.213, 205.
Toshchakov, V., et al. 2002.Nat. Immunol.3, 392.
Muller, M.R., et al. 2001.Immunology103, 49.
Vergne, I., and Cezanne, L. 1999.Eur. J. Biochem.264, 369.
Diaz-Guerra, M.J., et al. 1996.J. Biol. Chem.271, 30114.
Offermanns, S., et al. 1992.Biochem.J.282, 551.
Seifert, R., et al. 1990.Biochem.J.267, 795.
Hoffmann, P., et al. 1989.Biol. Chem. Hoppe.Seyler.370, 575.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Dan-Ning Hu et al.
Experimental eye research, 239, 109749-109749 (2023-12-20)
Pam3CSK4 activates Toll-like receptors 2 and 1 (TLR1/2), which recognize mainly molecules from gram-positive pathogens. The effect of Pam3CSK4 on various cytokine and chemokine expression in cultured human uveal melanocytes (UM) has not been studied systematically. The purpose of this
Irina Balan et al.
Translational psychiatry, 11(1), 145-145 (2021-02-28)
We have shown that endogenous neurosteroids, including pregnenolone and 3α,5α-THP inhibit toll-like receptor 4 (TLR4) signal activation in mouse macrophages and the brain of alcohol-preferring (P) rat, which exhibits innate TLR4 signal activation. The current studies were designed to examine
Shikha Nayar et al.
Nature, 593(7858), 275-281 (2021-04-01)
Crohn's disease is a chronic inflammatory intestinal disease that is frequently accompanied by aberrant healing and stricturing complications. Crosstalk between activated myeloid and stromal cells is critical in the pathogenicity of Crohn's disease1,2, and increases in intravasating monocytes are correlated
Wenjie Zhu et al.
bioRxiv : the preprint server for biology (2024-05-07)
The ocular surface is a mucosal barrier tissue colonized by commensal microbes, which tune local immunity by eliciting IL-17 from conjunctival γδ T cells to prevent pathogenic infection. The commensal Corynebacterium mastitidis (C. mast) elicits protective IL-17 responses from conjunctival
Arpan Acharya et al.
Journal of virology (2020-12-18)
HIV persists in cellular reservoirs despite effective combined antiretroviral therapy (cART) and there is viremia flare up upon therapy interruption. Opioids modulate the immune system and suppress antiviral gene responses, which significantly impact people living with HIV (PLWH). However, the
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门