生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat, mouse, canine, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pThr180/pTyr182)
基因信息
human ... MAPK14(1432)
一般描述
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
应用
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Immunocytochemistry (see comments)
警告
Toxicity: Standard Handling (A)
外形
In Dulbecco′s PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
其他说明
Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Marinissen, M.J., et al. 2001. Genes Dev.15, 535.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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