产品名称
Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3), liquid, clone CC22.8C7.3, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
CC22.8C7.3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2 µg/mL))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NOS1(4842)
General description
Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.
Analysis Note
Positive Control
Mouse macrophages treated with peroxynitrite
Mouse macrophages treated with peroxynitrite
Application
Immunoblotting (2 µg/ml)
Disclaimer
Toxicity: Standard Handling (A)
Immunogen
peroxynitrite-treated KLH
Other Notes
Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.
Physical form
In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
Preparation Note
Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Sanjeev Kumar et al.
The Journal of biological chemistry, 279(51), 53475-53482 (2004-10-01)
Plasmodium berghei invasion of Anopheles stephensi midgut cells causes severe damage, induces expression of nitric-oxide synthase, and leads to apoptosis. The present study indicates that invasion results in tyrosine nitration, catalyzed as a two-step reaction in which nitric-oxide synthase induction
Seungho Choi et al.
Neuroscience, 384, 41-53 (2018-05-22)
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Lalita Gupta et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(11), 4010-4015 (2005-03-09)
In vivo responses of midgut epithelial cells to ookinete invasion of three different vector-parasite combinations, Aedes aegypti-Plasmodium gallinaceum, Anopheles stephensi-Plasmodium berghei, and A. stephensi-P. gallinaceum, were directly compared by using enzymatic markers and immunofluorescence stainings. Our studies indicate that, in
Teresa Tiago et al.
Biochemistry, 45(11), 3794-3804 (2006-03-15)
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