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种属反应性
human
质量水平
制造商/商品名称
Milliplex®
技术
multiplexing: suitable
检测方法
fluorometric (Luminex® xMAP®)
储存温度
2-8°C
一般描述
NF-κB (nuclear factor kappa B) is a transcription factor that plays a regulatory role in several physiological and pathological processes. The NF-κB pathway can be activated by numerous stimuli, including inflammatory cytokines, virus, bacteria, UV radiation, genotoxic stress, DNA damage, oxidative stress, etc. The IkBα/NF-κB complex is tightly regulated by both phosphorylation and ubiquitination. Phosphorylation and ubiquitination of IκBα leads to its degradation and subsequent release of phosphorylated NF-κB to the nucleus. Greater than 100 target genes, including cytokines, cyclins, p21, Bcl-2, and c-Myc are known to be transcribed by NF-kB.
特异性
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
应用
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.This is an overnight (4°C) incubation assay.This kit must be run using the provided Assay Buffer.This assay requires 25 μL diluted filtered cell lysate per well.The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:c-Myc;FADD (Ser194);IκBα (Ser32);IKKα/β (Ser177/Ser181);NFκB (Ser536);TNFR1
包装
96-well plate
储存及稳定性
Recommended storage for kit components is 2 - 8°C.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
WGK
WGK 3
法规信息
新产品
相关内容
Multiplex assays simplify cancer research by measuring multiple biomarkers simultaneously for various signaling pathways.
MILLIPLEX® assays bridge immunology and cell signaling for inflammation studies, including T cell signaling and sepsis signaling.
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
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