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Merck
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安全信息

48-621MAG

Millipore

MILLIPLEX® DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay

别名:

Cell Signaling DNA Damage Kit, DNA Damage Bead Panel, Genotoxicity Magnetic Beads

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.47

种属反应性

mouse, human, rat

质量水平

制造商/商品名称

Milliplex®

技术

multiplexing: suitable

检测方法

fluorometric (Luminex® xMAP®)

储存温度

2-8°C

一般描述

The MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:• ATR (total)• Chk1 (Ser345)• Chk2 (Thr68)• H2A.X (Ser139)• MDM2 (total)• p21 (total)• p53 (Ser15)

特异性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

应用

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 1 (provided).The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:ATR (Total);Chk1 (Ser345);Chk2 (Thr68);H2A.X (Ser139);MDM2 (total);p21 (Total);p53 (Ser15)

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

法规信息

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相关内容

Exploring genotoxicity and DNA damage through multiplexing with MILLIPLEX® multiplex genotoxicity assays using Luminex® xMAP® technology enables the high-throughput measurement of phosphorylation levels of multiple proteins simultaneously and reduces sample volume, time, and cost.

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Multiplex toxicity assays enable researchers to simultaneously measure multiple toxicity biomarkers of various organs to gain a better understanding of toxicity. Explore how MILLIPLEX® multiplex toxicity assays are advancing research on liver injury, kidney toxicity, genotoxicity, and more.

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

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