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Merck
CN
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文件

安全信息

48-610MAG

Millipore

MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

别名:

Magnetic Bead STAT Kit, STAT Cell Signaling Kit

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.47

种属反应性

human

质量水平

200

制造商/商品名称

Milliplex®

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

储存温度

2-8°C

一般描述

The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.

特异性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

应用

  • An overnight (4°C) incubation is recommended for best results.
  • This assay requires 25 μL diluted cell lysate per well.
  • This kit must be run using Assay Buffer 1 (provided).
  • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).

Research Category
Signaling|Apoptosis & Cancer
The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)

包装

96-well plate

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

法律信息

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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