recombinant
expressed in E. coli
description
RTECS - QQ3450000
form
liquid
specific activity
≥40 units/mg protein
≥5 units/mL
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Disclaimer
毒性:有害(C)
General description
在大肠埃希菌中表达的重组产脲节杆菌 α2-3,6,8,9-神经氨酸酶。对来自复合碳水化合物和糖蛋白的全部非还原性末端唾液酸残基的水解进行催化。该唾液酸酶的高浓度和延长的培养期导致支链唾液酸的额外裂解。发现该酶是MW 60000和69000的两种活性物质。
注意:1 mU = 1毫单位。
Other Notes
Means, R.E. and Desrosiers, R.C.2000.J. Virol.74, 11181.
Prime, S., et al. 1996.J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995.Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993.Annu.Rev. Biochem.62, 65.
Prime, S., et al. 1996.J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995.Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993.Annu.Rev. Biochem.62, 65.
一个单位定义为在37°C,pH值5.0下每分钟催化从2ʹ-(4-甲基伞形酮基)-α-D-N-乙酰神经氨酸释放1µ摩尔甲基伞形酮的酶量。(注意:1 mU = 1毫单位)。
Physical form
在20 mM Tris-HCl,150 mM NaCl,pH 7.5中。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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