质量水平
检测方案
≥98% (silver nitrate titration)
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
杂质
≤0.5% Sulfated ash
颜色
yellow
溶解性
PBS: 2 mg/mL
痕量阳离子
heavy metals: ≤0.001%
运输
ambient
储存温度
15-25°C
InChI
1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI key
AZKSAVLVSZKNRD-UHFFFAOYSA-M
相关类别
一般描述
一种膜渗透性黄色染料,在活细胞中被线粒体还原酶还原形成深蓝色产物MTT-甲臜。MTT-甲臜在540或570 nm处的分光光度测量可定量细胞活力。用作细胞毒性(如用于筛选癌症药物)和凋亡的直接指标。一种用于研究NADP依赖性脱氢酶类的电子受体。
生化/生理作用
主要靶标
用作细胞毒性和凋亡的直接指标。
用作细胞毒性和凋亡的直接指标。
产物不与ATP竞争。
可逆:否
细胞可渗透性:具有
警告
毒性:有害 & 致癌/致畸性(E)
重悬
复溶后,分装并冷冻保存(-20°C)。贮备液在-20°C下可稳定保存至多1个月。
其他说明
Liu, Y., et al. 1997.J. Neurochem.69, 581.
Abdallah, M.A. 1980.Eur. J. Biochem.112, 331.
Abdallah, M.A. 1980.Eur. J. Biochem.112, 331.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Warning
危险分类
Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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