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Merck
CN
所有图片(1)

文件

475989

Sigma-Aldrich

噻唑蓝四唑蓝

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

别名:

噻唑蓝四唑蓝, 3-(4,5-二甲基-2-噻唑)-2,5-二苯基-2H-溴化四唑噻唑蓝

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About This Item

经验公式(希尔记法):
C18H16BrN5S
CAS号:
分子量:
414.32
MDL编号:
UNSPSC代码:
12171500
NACRES:
NA.25

质量水平

检测方案

≥98% (silver nitrate titration)

形式

solid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

杂质

≤0.5% Sulfated ash

颜色

yellow

溶解性

PBS: 2 mg/mL

痕量阳离子

heavy metals: ≤0.001%

运输

ambient

储存温度

15-25°C

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

相关类别

一般描述

一种膜渗透性黄色染料,在活细胞中被线粒体还原酶还原形成深蓝色产物MTT-甲臜。MTT-甲臜在540或570 nm处的分光光度测量可定量细胞活力。用作细胞毒性(如用于筛选癌症药物)和凋亡的直接指标。一种用于研究NADP依赖性脱氢酶类的电子受体。

生化/生理作用

主要靶标
用作细胞毒性和凋亡的直接指标。
产物不与ATP竞争。
可逆:否
细胞可渗透性:具有

警告

毒性:有害 & 致癌/致畸性(E)

重悬

复溶后,分装并冷冻保存(-20°C)。贮备液在-20°C下可稳定保存至多1个月。

其他说明

Liu, Y., et al. 1997.J. Neurochem.69, 581.
Abdallah, M.A. 1980.Eur. J. Biochem.112, 331.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazardExclamation mark

警示用语:

Warning

危险分类

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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