形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
颜色
yellow
储存温度
10-30°C
InChI
1S/C2H4O/c1-2-3/h2-3H,1H2
InChI key
IMROMDMJAWUWLK-UHFFFAOYSA-N
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一般描述
Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
警告
Toxicity: Standard Handling (A)
制备说明
Add 2.4 g of MOWIOL® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.
重悬
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
其他说明
Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.
Beug, H., et al. 1979. Cell18, 375.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Mowiol is a registered trademark of Kuraray Europe GmbH
WGK
WGK 1
法规信息
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