产品名称
Anti-Mast Cell Tryptase Mouse mAb (AA1), liquid, clone AA1, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
AA1, monoclonal
form
liquid
does not contain
preservative
species reactivity
monkey, canine, human, feline
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (0.06-0.12 µg/mL)
Immunoblotting (1 µg/mL)
Paraffin Sections (0.06-0.12 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TPSAB1(7177)
Analysis Note
Positive Control
Cat, dog, human, or monkey mast cells
Cat, dog, human, or monkey mast cells
Application
Frozen Sections (0.06-0.12 µg/ml)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.
Immunogen
Human
native, human mast cell, tryptase
Other Notes
Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.
Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Johannes Kapeller et al.
The Journal of comparative neurology, 519(3), 420-432 (2010-12-31)
Since the first description of 5-HT₃ receptors more than 50 years ago, there has been speculation about the molecular basis of their receptor heterogeneity. We have cloned the genes encoding novel 5-HT3 subunits 5-HT3C, 5-HT3D, and 5-HT3E and have shown
Henry R Maun et al.
Cell, 179(2), 417-431 (2019-10-05)
Severe asthma patients with low type 2 inflammation derive less clinical benefit from therapies targeting type 2 cytokines and represent an unmet need. We show that mast cell tryptase is elevated in severe asthma patients independent of type 2 biomarker
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