Anti-Hsp90 Rat mAb (16F1)

Protein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Rat mAb (16F1) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Electron Microscopy for the detection of Hsp90.

Hsp90 isolated from therapeutic human orchiectomy specimens

Human

ELISA (see comments)

Immunoblotting (2 µg/ml, colormetric)

Immunocytochemistry (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Electron Microscopy (see comments)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In PBS, 50% glycerol.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Heat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells

Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
Lanks, K.W., et al. 1982. J. Biol. Chem.257, 8604.

This antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.

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