推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
AC88, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... HSP90AA1(3320)
一般描述
Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.
免疫原
Achyla ambisexualis
Hsp90 isolated from water mold, Achyla ambisexualis
应用
Immunoblotting (1 µg/ml, colorimetric)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 10 mM PBS, 50% glycerol, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
其他说明
Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Bahram Samanfar et al.
PeerJ, 5, e4037-e4037 (2017-11-22)
The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been
Elevated levels of ribosomal proteins eL36 and eL42 control expression of Hsp90 in rhabdomyosarcoma.
Sarah Shaikho et al.
Translation (Austin, Tex.), 4(2), e1244395-e1244395 (2017-01-17)
Mammalian 90 kDa heat shock protein (Hsp90) is a ubiquitous molecular chaperone whose expression is selectively upregulated during stress, although the precise control mechanism of this increase is yet to be fully elucidated. We used polysome profiling to show that Hsp90α
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Life science alliance, 3(12) (2020-10-25)
Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular
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Epigenetics, 12(11), 953-963 (2017-09-28)
Estrogen receptors (ESR1 and ESR2) play crucial roles in various processes during spermatogenesis. To elucidate individual roles of ESRs in male fertility, we developed in vivo selective ESR agonist administration models. Adult male rats treated with ESR1 and ESR2 agonist
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