recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥10,000 units/mL, ≥5,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
molecular cloning: suitable
input
purified RNA
foreign activity
Nuclease-free
shipped in
wet ice
storage temp.
−20°C
General description
在大肠埃希菌中表达的重组HIV逆转录酶。催化DNA和RNA模板上容易出错的合成。为评估抗病毒药物提供了一个极好的靶标。
Application
大肠杆菌来源重组HIV 逆转录酶可用作逆转录酶活性测定标准品。
Packaging
请参考特定浓度批号的标签。
Physical form
在10 mM磷酸钾,1 mM DTT,20%甘油(pH 7.4)中。
Preparation Note
初次融化后,等分并冷冻(-20°C)。
Other Notes
一个单位定义为在37°C,pH 8.3下20分钟内将1.0 nmol标记的dTTP掺入酸不溶性物质中的酶量。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
存储类别
10 - Combustible liquids
wgk
WGK 1
Geraldine Vilmen et al.
mBio, e0275221-e0275221 (2022-01-12)
Infection of rhesus macaques with simian-human immunodeficiency viruses (SHIVs) is the preferred model system for vaccine development because SHIVs encode human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Envs)-a key target of HIV-1 neutralizing antibodies. Since the goal of vaccines
Conformation of HIV-1 Envelope governs rhesus CD4 usage and simian-human immunodeficiency virus replication
Vilmen G, et al.
mBio, 13(1), e02752-e02721 (2022)
Identification of an antiretroviral small molecule that appears to be a host-targeting inhibitor of HIV-1 assembly
Reed JC, et al.
Journal of Virology, 95(3), 10-1128 (2021)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 382129-500U | 04055977213140 |