质量水平
检测方案
≥95% (size exclusion chromatography)
形式
liquid
制造商/商品名称
Calbiochem®
储存条件
do not freeze
杂质
≤25 EU/mg Endotoxin
≥1.5 x 10⁻³ copper to protein ratio
≥5 mg/mL Protein
颜色
clear bluish
运输
wet ice
储存温度
2-8°C
一般描述
匙孔血蓝蛋白（KLH）是一种大型的多亚基、携氧金属蛋白。这种高分子量糖蛋白是由海洋蜗牛大锁孔帽贝提取而来。
应用
来自大锁孔帽贝的匙孔血蓝蛋白，高纯度，无内毒素，无菌液体，已作为抗原用于通过酶联免疫吸附剂测定（ELISA）测试单克隆抗体（mAb）的多反应性。它也已用于使动物免疫以研究猴子中的KLH特异性体外 回忆反应（recall response）和特异性T细胞依赖性抗体反应（TDAR）。
生化/生理作用
匙孔血蓝蛋白（KLH）刺激动物和人类中细胞介导的反应和体液反应。它诱发免疫刺激特性并可作为疫苗佐剂。KLH可作为载体蛋白用于生产抗体和半抗原，如肽和寡糖。它也可用于治疗膀胱癌。
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
在83 mM磷酸钠，150 mM NaCl（pH 7.2）中。
制备说明
KLH是一种极大的蛋白复合物，具有在溶液中聚集和沉淀的趋势。 稀释KLH时使用缓冲液，并将镁浓度保持在≥10 mM。 KLH 溶液在 4°C下储存。切勿冷冻、涡旋处理或搅拌以溶解KLH。冷冻或搅拌KLH将导致蛋白质聚集和沉淀。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it
Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Joshua T Gamse et al.
Toxicology and applied pharmacology, 409, 115285-115285 (2020-10-19)
The OX40 receptor plays a crucial co-stimulatory role in T effector cell survival, expansion, cytokine production, and cytotoxicity to tumor cells; therefore, OX40 agonists are being evaluated as anti-cancer immunotherapies, especially in combination with checkpoint inhibitors. To support clinical development
Jenna J Guthmiller et al.
Immunity, 53(6), 1230-1244 (2020-10-24)
Polyreactivity is the ability of a single antibody to bind to multiple molecularly distinct antigens and is a common feature of antibodies induced upon pathogen exposure. However, little is known about the role of polyreactivity during anti-influenza virus antibody responses.
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