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主要文件

371720

Sigma-Aldrich

Anti-Giα-1-Subunit, Internal (159-168) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

diluted serum

抗体产品类型

primary antibodies

克隆

polyclonal

表单

liquid

不包含

preservative

种属反应性

mouse

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG

运输

wet ice

储存温度

−70°C

靶向翻译后修饰

unmodified

基因信息

mouse ... Gnai1(14677)

一般描述

Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-1 subunit.
Recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits.

免疫原

a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse Giα-1, conjugated to KLH

应用

Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外形

Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, Goα and Gsα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Hiroko Ohtsuka et al.
PloS one, 12(1), e0169609-e0169609 (2017-01-11)
Stromal cell-derived factor-1α (SDF-1α)-induced platelet aggregation is mediated through its G protein-coupled receptor CXCR4 and phosphatidylinositol 3 kinase (PI3K). Here, we demonstrate that SDF-1α induces phosphorylation of Akt at Thr308 and Ser473 in human platelets. SDF-1α-induced platelet aggregation and Akt
Yatin M Patel et al.
Blood, 101(12), 4828-4835 (2003-03-01)
We have examined platelet functional responses and characterized a novel signaling defect in the platelets of a patient suffering from a chronic bleeding disorder. Platelet aggregation responses stimulated by weak agonists such as adenosine diphosphate (ADP) and adrenaline were severely
H-D Yan et al.
British journal of pharmacology, 139(3), 605-615 (2003-06-06)
(1) The effects on the whole-cell carbachol-induced muscarinic cationic current (mIcat) of antibodies against the alpha-subunits of various G proteins, as well as the effect of a Gbetagamma subunit, were studied in single guinea-pig ileal smooth muscle cells voltage-clamped at
Takashi Hirakawa et al.
Endocrinology, 144(9), 3872-3878 (2003-08-23)
Using a Leydig tumor cell line (MA-10) transiently transfected with the human lutropin receptor (hLHR) and mutants thereof, we examined the identity of the G proteins activated by the agonist-engaged hLHR-wild type (wt) and by three of its naturally occurring
Shannon M Gifford et al.
The Journal of endocrinology, 190(2), 373-384 (2006-08-11)
Uterine artery endothelial cells (UAEC) derived from pregnant (P-UAEC) and nonpregnant (NP-UAEC) ewes retain pregnancy-specific differences in cell signaling as well as vasodilator production through passage 4. In particular, when P- and NP-UAEC are stimulated with ATP over a 2.5

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