推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2D3, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative (antibody only)
种属反应性
mouse, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer9)
基因信息
human ... GSK3B(2932)
mouse ... Gsk3B(56637)
一般描述
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
应用
ELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
Immunoblotting (0.5 µg/ml)
警告
Toxicity: Standard Handling (A)
外形
100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
重悬
Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
分析说明
Positive Control
Pervanadate-treated HepG2 (included)
Pervanadate-treated HepG2 (included)
其他说明
For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Methods in molecular biology (Clifton, N.J.), 468, 45-65 (2008-12-23)
Glycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action ofWnts. This protein kinase is ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling or activation of growth factor receptor tyrosine kinases
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