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重组
expressed in E. coli
质量水平
检测方案
≥90% (SDS-PAGE)
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
运输
wet ice
储存温度
−70°C
一般描述
Recombinant, human GSK-3β fused at the N-terminus to a His•Tag sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer’s disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.
Recombinant, human GSK-3β fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer′s disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB. MW: 53,000-56,000
生化/生理作用
1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from 0.1 M NH₄CO₃.
重悬
Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute in sterile PBS to a final concentration of 1 mg/ml.
其他说明
Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol.5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Genes & development, 22(2), 252-264 (2008-01-17)
Peroxisome proliferator-activated receptor gamma (PPARgamma) coactivator-1alpha (PGC-1alpha) is a highly regulated transcriptional coactivator that coordinates energy metabolism in mammals. Misregulation of PGC-1alpha has been implicated in the pathogenesis of several human diseases, including diabetes, obesity, and neurological disorders. We identified
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