重组
expressed in E. coli
质量水平
检测方案
≥95% (SDS-PAGE)
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
杂质
<1.0 EU/μg Endotoxin (EU/μg EGF)
运输
ambient
储存温度
−20°C
一般描述
在大肠埃希菌中表达的重组人表皮生长因子（54个氨基酸）。 除了一个额外的N端蛋氨酸，与天然人EGF相同。人EGF与小鼠EGF同源性为70%，并已显示具有相似的生物学活性。对来自组织（例如乳房，角膜，表皮，真皮，肝脏，胰腺，神经，羊膜和肾上腺髓质）的多种外胚层和内胚层来源的细胞具有促有丝分裂作用。EGF与其170 kDa跨膜受体的结合导致受体的二聚化，内在受体酪氨酸激酶的激活以及导致细胞增殖和/或分化的复杂信号转导级联反应的启动。用作在预防细胞凋亡中的存活因子。
在大肠埃希菌中表达的重组人表皮生长因子（54个氨基酸）。除了一个额外的N端蛋氨酸，与人EGF相同。人EGF与小鼠EGF同源性为70%，并已显示具有相似的生物学活性。对来自组织（例如乳房，角膜，表皮，真皮，肝脏，胰腺，神经，羊膜和肾上腺髓质）的多种外胚层和内胚层来源的细胞具有促有丝分裂作用。用作在预防细胞凋亡中的存活因子。
生化/生理作用
ED₅₀ = 100-400 pg/ml，通过其刺激EGF反应性小鼠成纤维细胞系Balb/3T3中³H-胸苷掺入的能力来衡量。
警告
毒性：标准处理（A）
重悬
复溶后，等分并冷冻长期保存（-20°C或-70°C），或冷藏（4°C）短期保存。避免溶液反复冻融。储备溶液在4°C下可稳定保存1个月，在-20°C或-70°C下可稳定保存3个月。
用含有≥0.1％HSA或BSA的10 mM乙酸复溶成浓度≥10 µg/ml的溶液。
其他说明
Merlo, G.R., et al. 1995.J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993.Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991.Proc.Natl.Acad.Sci. USA88, 415.
Carpenter, G., and Wahl, M.I.1990.In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.)Springer-Verlag, New York, p 69.
Downward, J., et al. 1984.Nature311, 483.
Gray, A., et al. 1983.Nature303, 722.
Scott, J., et al. 1983.Science221, 236.
Cohen, S. 1960.Proc.Natl.Acad.Sci. USA46, 302.
Yoshida, T., et al. 1993.Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991.Proc.Natl.Acad.Sci. USA88, 415.
Carpenter, G., and Wahl, M.I.1990.In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.)Springer-Verlag, New York, p 69.
Downward, J., et al. 1984.Nature311, 483.
Gray, A., et al. 1983.Nature303, 722.
Scott, J., et al. 1983.Science221, 236.
Cohen, S. 1960.Proc.Natl.Acad.Sci. USA46, 302.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Eric Okerberg et al.
Current protocols in chemical biology, 5(3), 213-226 (2014-01-07)
The protocols in this unit describe efficient and cost-effective approaches to determine the interaction of small-molecule inhibitors with native kinases, and also analyze the interactions between kinases and their binding partners in a cellular setting. The combined attributes of activity-based
Katarzyna A Ludwik et al.
Cell reports, 32(3), 107931-107931 (2020-07-23)
In response to estrogens, estrogen receptor alpha (ERα), a critical regulator of homeostasis, is degraded through the 26S proteasome. However, despite the continued presence of estrogen before menopause, ERα protein levels are maintained. We discovered that ERK1/2-RSK2 activity oscillates during
Marta Grońska-Pęski et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(13), 2899-2910 (2021-02-28)
The addition of new neurons to existing neural circuits in the adult brain remains of great interest to neurobiology because of its therapeutic implications. The premier model for studying this process has been the hippocampal dentate gyrus in mice, where
Lindsey Seldin et al.
Developmental cell, 55(5), 558-573 (2020-10-16)
DNA crosslinking agents are commonly used in cancer chemotherapy; however, responses of normal tissues to these agents have not been widely investigated. We reveal in mouse interfollicular epidermal, mammary and hair follicle epithelia that genotoxicity does not promote apoptosis but
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门