species reactivity
guinea pig, rabbit, pig, mouse, horse, rat, canine, nonhuman primates, hamster, human
packaging
pkg of 1000 assay(s)
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
detection method
colorimetric
Quality Level
Application
The BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The CHEMICON® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.
Test Principle
Within CHEMICON®′s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.
For Research Use Only. Not for use in diagnostic procedures.
Test Principle
Within CHEMICON®′s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.
For Research Use Only. Not for use in diagnostic procedures.
Other Notes
- BrdU Reagent: (Part No. 2752a) One 100 μL vial of a 500X solution of BrdU.
- Fixing Solution: (Part No. 2752b) One 200 mL bottle.
- Prediluted BrdU Detection Antibody: (Part No. 2752c) One 100 mL vial of anti-BrdU in solution.
- Stop Solution: (Part No. 2752d): One 100 mL bottle of 2.5N sulfuric acid solution.
- Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2752e) One 60 μL vial of a 2000X solution.
- Conjugate Diluent: (Part No. 2752f) One 100 mL bottle.
- Substrate: (Part No. 2752g) One 100 mL bottle, ready to use TMB.
- 50X Plate Wash Concentrate: (Part No. 2752h) One 125 mL bottle of buffered Tris with surfactant.
Packaging
1000 assays
Preparation Note
Maintain the unopened kit at 2-8°C until the expiration date indicated on the label.
Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution
Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
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