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Merck
CN
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文件

251520-M

Sigma-Aldrich

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

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别名:
DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
经验公式（希尔记法）:
C25H18F2N2O7
CAS号:
254109-22-3
分子量:
496.42
MDL编号:
MFCD16872050
UNSPSC代码:
12352200
NACRES:
NA.25

质量水平

100

检测方案

≥98% (HPLC)

形式

solution

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
protect from light

颜色

pale yellow

荧光

λex ~500 nm
λem ~515 nm

储存温度

−20°C

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

一般描述

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

生化/生理作用

Primary Target
Nitric oxide (NO) fluorescent indicator

警告

Toxicity: Irritant (B)

外形

A 5 mM solution in DMSO.

重悬

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

其他说明

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

法律信息

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

188.6 °F

闪点(°C)

87 °C

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