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Merck
CN
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文件

251500-M

Sigma-Aldrich

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

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别名:
DAF-2, 4,5-Diaminofluorescein
经验公式(希尔记法):
C20H14N2O5
分子量:
362.34
MDL编号:
UNSPSC代码:
12171500
NACRES:
NA.25

质量水平

检测方案

≥98% (HPLC)

形式

solution

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
protect from light

颜色

pale yellow

荧光

λex ~495 nm
λem ~515 nm

储存温度

−20°C

InChI

1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2

InChI key

LTYUPYUWXRTNFQ-UHFFFAOYSA-N

一般描述

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

生化/生理作用

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

警告

Toxicity: Irritant (B)

外形

A 5 mM (1 mg in 550 µl) solution in DMSO.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

法律信息

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2


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