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重组
expressed in E. coli
质量水平
检测方案
≥95% (SDS-PAGE)
形式
liquid
比活
≥1450 units/μg protein
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
运输
wet ice
储存温度
−70°C
一般描述
M.W. 12,000 and 17,000 (heterodimer).
Recombinant, human caspase-3 expressed in E. coli as the proenzyme, which is subsequently cleaved to produce the active enzyme. Suitable for the study of enzyme regulation, kinetics, and target substrate cleavage and inhibitor screening. MW: 12000 and 17000.
Recombinant, human caspase-3 expressed in E. coli as the proenzyme, which is subsequently cleaved to produce the active enzyme. The active enzyme is a heterodimer with subunit molecular weights of 12 kDa and 19 kDa. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage and inhibitor screening. Caspase-3 is a member of the ICE family of cysteine proteases that is activated during apoptotic signaling events by upstream proteases, including caspase-6, caspase-8 (FLICE) and cytotoxic T-cell-derived granzyme B.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will cleave 1.0 pmol of the substrate Ac-DEVD-pNA (Cat. No. 235400) per min at 30°C, pH 7.4.
外形
In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Izban, K.F., et al. 1999. Am. J. Pathol. 154, 1439.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Mittl, P.R.E., et al. 1997. J. Biol. Chem. 272, 6539.
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Mittl, P.R.E., et al. 1997. J. Biol. Chem. 272, 6539.
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
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