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Merck
CN
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文件

219461

Sigma-Aldrich

组织蛋白酶K，His•Tag®，人，重组，大肠埃希菌

Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

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CAS号:
94716-09-3
EC 号:
3.4.22.38 (BRENDA, IUBMB)
UNSPSC代码:
12352202
NACRES:
NA.77

重组

expressed in E. coli

质量水平

100

检测方案

≥95% (SDS-PAGE)

形式

liquid

比活

≥900 mU/mg protein

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−70°C

一般描述

注意：1 mU = 1百万单元。
组织蛋白酶K是半胱氨酸蛋白酶木瓜蛋白酶超家族的成员，在破骨细胞介导的骨吸收和胶原降解中起重要作用。它主要由破骨细胞表达。最近报道也在成纤维细胞中表达。活性异常会导致骨质减少性疾病，例如骨质疏松症症和致密性成骨不全症症。包含C-端His•Tag。
组织蛋白酶K是半胱氨酸蛋白酶木瓜蛋白酶超家族的成员，在破骨细胞介导的骨吸收和胶原降解中起重要作用。它主要由破骨细胞表达，最近的研究也表明它在成纤维细胞中表达。组织蛋白酶K活性异常，无论是太高还是太低，都会导致骨质减少性疾病，例如骨质疏松症症和致密性成骨不全症症。该重组酶包含C端His•Tag序列。

包装

请参考特定浓度批号的标签。

警告

毒性：标准处理（A）

单位定义

一个单位定义为在37°C，pH 5.5下，每分钟水解0.4 µmol Z-FR-AMC的酶量。

外形

在50 mM NaOAC，pH 5.5中。通过添加10 mM MMTS停止激活，可以在还原条件下将其去除。

重悬

初次解冻后，分装冻存（-70°C）。

其他说明

Delaisse, J.M., et al. 2003.Microsc.Res. Tech.61, 504.
Dodds, R.A.2003.Cell Biochem.Funct.21, 231.
Everts, V., et al. 2003.Calcif.Tissue Int.73, 380.
Hou, W.S., et al. 2003.Biol. Chem.384, 891.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

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