218807
Caspase-9, Human, Recombinant, E. coli
Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
别名:
ICE-LAP6, Mch6
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About This Item
UNSPSC代码:
12352202
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重组
expressed in E. coli
质量水平
方案
≥70% (SDS-PAGE)
表单
lyophilized
比活
≥400 units/mg protein
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
溶解性
PBS with 15% glycerol: 1 units/μL
运输
wet ice
储存温度
−70°C
一般描述
Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.
外形
Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
重悬
Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
其他说明
Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
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