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The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
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主要文件

218800

Sigma-Aldrich

Caspase-7, Human, Recombinant, E. coli

Caspase-7, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

别名:

Mch3, ICE-LAP3

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50 EA
CN¥4,855.76

CN¥4,855.76


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50 EA
CN¥4,855.76

About This Item

UNSPSC代码:
12352202

CN¥4,855.76


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重组

expressed in E. coli

质量水平

表单

lyophilized

比活

≥15,000 units/mg protein

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−70°C

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1 of 4

此商品
SAB1411370SAB1406563SAB1409776
biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

western blot: 1 μg/mL

technique(s)

indirect immunofluorescence: suitable, western blot: 1 μg/mL

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human

species reactivity

human

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

2G12, monoclonal

一般描述

M.W. 12,000 and 20,000 (heterodimer).
Recombinant, human caspase-7 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.

重悬

Following reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.

其他说明

Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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