218799
Caspase-6, Human, Recombinant, E. coli
Caspase-6, Human, Recombinant is expressed as a single polypeptide in E. coli. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
别名:
Mch2
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352202
Specific activity:
≥6000 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized
specific activity
≥6000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.
Other Notes
LeBlanc, A., et al. 1999. J. Biol. Chem.274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Chunyan Wang et al.
Neuroscience letters, 721, 134802-134802 (2020-02-06)
Neuroinflammation in the spinal cord is a pathological event in remifentanil-induced hyperalgesia (RIH), but its underlying molecular mechanisms remain unclear. Recent studies recapitulate the significance of the intracellular protease caspase-6 in the release of inflammatory mediators and synaptic plasticity in
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