218799
Caspase-6, Human, Recombinant, E. coli
Caspase-6, Human, Recombinant is expressed as a single polypeptide in E. coli. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
别名:
Mch2
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About This Item
UNSPSC代码:
12352202
NACRES:
NA.77
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重组
expressed in E. coli
质量水平
方案
≥95% (SDS-PAGE)
表单
lyophilized
比活
≥6000 units/mg protein
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
运输
wet ice
储存温度
−70°C
一般描述
M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.
重悬
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.
其他说明
LeBlanc, A., et al. 1999. J. Biol. Chem.274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Chunyan Wang et al.
Neuroscience letters, 721, 134802-134802 (2020-02-06)
Neuroinflammation in the spinal cord is a pathological event in remifentanil-induced hyperalgesia (RIH), but its underlying molecular mechanisms remain unclear. Recent studies recapitulate the significance of the intracellular protease caspase-6 in the release of inflammatory mediators and synaptic plasticity in
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