218769
Caspase-8, Human, Recombinant, E. coli
Caspase-8, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
别名:
FLICE, MACH, Mch5
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About This Item
UNSPSC代码:
12352202
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重组
expressed in E. coli
质量水平
方案
≥90% (SDS-PAGE)
表单
liquid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
运输
wet ice
储存温度
−70°C
一般描述
M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-8 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4.
外形
In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-70°).
其他说明
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
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