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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
IB4, monoclonal
方案
≥95% (SDS-PAGE)
表单
liquid
包含
0.5 mg/mL gentamicin sulfate as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
抗-CD18,小鼠单克隆,克隆IB4,可识别~95 kDa β2整联蛋白亚基。它已针对冷冻切片上的 FC、IP、IHC和细胞抑制试验进行了验证。
蛋白A纯化的小鼠单克隆抗体。识别~95 kDa β2整联蛋白亚基(CD18)。
识别~95 kDa β2整联蛋白亚基。阻断ICAM-1和ICAM-3与LFA-1的结合。
免疫原
人
人外周血血单核细胞
应用
流式细胞术(5-10 µg/ml)
冷冻切片(参见注释)
免疫沉淀(参见注释)
细胞抑制试验(参见注释)
冷冻切片(参见注释)
免疫沉淀(参见注释)
细胞抑制试验(参见注释)
包装
请参考特定浓度批号的标签。
警告
毒性:标准处理(A)
外形
在150 mM NaCl,100 mM KCl,50 mM磷酸钠中,pH 7.5。
重悬
初次使用后,分装冻存(-20°C)以进行长期储存。避免冻/融循环。
分析说明
阳性对照
HPB-MLT细胞
HPB-MLT细胞
阴性对照
Nalm-6细胞
Nalm-6细胞
其他说明
Berman, M.E., et al. 1996.J. Immunol.156, 1515.
Kaneko, M., et al. 1995.J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995.Haematologica80, 161.
Kaneko, M., et al. 1995.J. Immunol.155, 2631.
Landis, R.C., et al. 1994.J. Cell Biol.126, 529.
Wright, S.D., et al. 1983.Proc.Natl.Acad.Sci. USA80, 5699.
Kaneko, M., et al. 1995.J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995.Haematologica80, 161.
Kaneko, M., et al. 1995.J. Immunol.155, 2631.
Landis, R.C., et al. 1994.J. Cell Biol.126, 529.
Wright, S.D., et al. 1983.Proc.Natl.Acad.Sci. USA80, 5699.
阻断ICAM-1和ICAM-3与LFA-1的结合。使用5 µg/ml的抗-CD18抗体和FITC偶联的山羊抗小鼠IgG对5x105培养的人HPB-MLT细胞进行FACS。据报道,该抗体也可用于免疫沉淀和冰冻切片。合适的工作稀释度由与测定条件相关的变量决定。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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