推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
UCHT1, monoclonal
检测方案
>95% (immunoglobulin, SDS-PAGE)
形式
liquid
包含
0.5 mg/mL gentamycin sulfate as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
抗CD3，小鼠单克隆，克隆UCHT1，可识别人外周T细胞中CD3复合物的ε-链。已针对冰冻切片的FC，IP，IHC和T细胞共刺激进行了验证。
蛋白A纯化的小鼠单克隆抗体。识别CD3 T细胞受体复合物的~20 kDaε链。
识别人外周T细胞中CD3复合物的ε-链。可用于激活表达CD3ε的T细胞。
免疫原
人胸腺细胞/Sezary T细胞
应用
流式细胞术（5 µg/ml）
冷冻切片（参见备注）
免疫沉淀法（参见备注）
T细胞共刺激（参见应用参考）
冷冻切片（参见备注）
免疫沉淀法（参见备注）
T细胞共刺激（参见应用参考）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
其他说明
Thibault, G. and Bardos, P. 1995.J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E.1981.Eur. J. Immunol.11, 329.
Beverley, P.C., and Callard, R.E.1981.Eur. J. Immunol.11, 329.
该抗体可用于激活表达CD3ε的T细胞（见应用参考）。对5×105培养的HPB-MLT人肿瘤细胞进行FACS。当与二级试剂山羊抗小鼠IgG（FITC偶联）一起使用时，测试的最佳结合浓度为5 µg/mL。据报道，该抗体可用于免疫沉淀和冷冻切片染色。合适的工作稀释度由与测定条件相关的变量决定。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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