推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
FMC75, monoclonal
形式
liquid
不包含
preservative
种属反应性
human, monkey
请勿与下列物质发生反应
rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
免疫原
Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein
应用
Enzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In PBS, 50% Glycerol and 0.09% Sodium Azide.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
其他说明
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Stephanie S Lam et al.
Nature methods, 12(1), 51-54 (2014-11-25)
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Ying Yin et al.
PLoS biology, 16(8), e2005140-e2005140 (2018-08-08)
Some secreted proteins that assemble into large complexes, such as extracellular matrices or hormones and enzymes in storage granules, must be kept at subaggregation concentrations during intracellular trafficking. We show surfeit locus protein 4 (Surf4) is the cargo receptor that
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Wang, et al.
International Journal of Molecular Sciences, 24 (2023)
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