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Merck
CN
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文件

19-160

Sigma-Aldrich

BrdU（5-溴-2′-脱氧尿苷）

Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine, BrdU can be used in conjunction with anti-BrdU for immunocytochemical analysis of cell proliferation.

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UNSPSC代码:
41116012
eCl@ss:
32160405
NACRES:
NA.41

质量水平

100

制造商/商品名称

Upstate®

技术

immunocytochemistry: suitable

运输

wet ice

应用

用于DNA合成研究，将其代替胸腺嘧啶脱氧掺入DNA；BrdU可与抗BrdU一起用于细胞增殖的免疫细胞化学分析。
研究范畴
全部

外形

C9H11BrN2O5

储存及稳定性

在-20°C下可保存2年

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H340,H361fd

危险分类

Muta. 1B - Repr. 2

WGK

WGK 2

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is contained in Product 19160, SOD Assay Kit (Superoxide dismutase)?

    This kit contains WST Solution (1x5mL), Enzyme Solution (1x100μL), Buffer Solution (1x100mL), Dilution Buffer (1x50mL), and a manual.  

  4. What equipment is required to use Product 19160, SOD Assay Kit (Superoxide dismutase)?

    The equipment needed to use this kit is a plate reader (450 nm filter), a 96-well microplate, 10 μL & 100-200 μL pipettes, a multichannel pipette and an incubator. Superoxide dismutase (SOD) is also necessary for the preparation of an inhibition curve.

  5. How do I prepare a WST working solution for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    Dilute 1mL of WST Solution with 19mL of Buffer Solution.

  6. When using Product 19160, SOD Assay Kit (Superoxide dismutase), how do I prepare an Enzyme working solution?

    Centrifuge the Enzyme Solution tube for five seconds.  Mix by pipeting, and dilute 15μL of Enzyme Solution with 2.5mL of Dilution Buffer.

  7. When using Product 19160, SOD Assay Kit, how do I prepare a Superoxide Dismutase (SOD) working solution?

    Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows:  200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.

  8. Are there any literature references for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    1. J. M. McCord and I. Fridovich, J. Biol. Chem., 244, 6049 (1969).  2. S. Goldstein and G. Czapski, Free Rad. Res. Comms.,12-13(Part 1), 5-10 (1991).  3. R. H. Burdon, V. Gill, and C. Rice-Evans, Free Rad. Res. Comms., 18, 369 (1993).  4. M. W. Sutherland and B. Learmonth, Free Rad. Res., 27, 283 (1997).  5. H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).  6. H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, Biosci. Biotechnol. Biochem., 63, 485 (1999).  7. A. V. Peskin, et al., Clin. Chim. Acta., 293, 157 (2000).

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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