Arp2/3 Complex Inhibitor II, CK-869

A cell-permeable thiazolidinone compound that inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human and bovine, but not yeast, species (IC₅₀ = 11 µM against bovine Arp2/3). Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC₅₀ = 7 µM). Structrual studies indicate that CK-869 targets the hydrophobic core in subdomain 1 of Arp3. CK-666 (Cat. No. 182515) and CK-869 exhibit different modes of binding, resulting in their different yeast cross-reactivities. Supplied as a racemic mixture of the active 2S and the inactive 2R enantiomers. CK-312 (Cat. No. 182518) can serve as a negative control.

A cell-permeable thiazolidinone compound that inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human and bovine, but not yeast, species (IC₅₀ = 11 µM against bovine Arp2/3). Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC₅₀ = 7 µM). Structural studies indicate that CK-869 targets the hydrophobic core in subdomain 1 of Arp3. CK-666 and CK-869 exhibit different modes of binding, resulting in their different yeast cross-reactivities. Supplied as a racemic mixture of the active 2S and the inactive 2R enantiomers. CK-312 (Cat. No. 182518) can serve as a negative control.

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Nolen, B.J., et al. 2009. Nature460, 1031.

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