一般描述
亚基结构：作用于SIN3A、SIN3B和RCOR1。
亚细胞定位：细胞核（可能）。
组织特异性：普遍分布。在淋巴细胞室组织(lymphocytic compartment)（包括脾、胸腺、周围血液淋巴细胞和卵巢）中表达水平很高。
序列相似性：含有9个C2H2型锌指结构。
序列警告：AAA98503.1与所示不同。原因：189、212和1087位点发生移码。
AAH38985.1序列与所示不同。原因：其他差异。污染序列。可能的poly-A序列。
应用
生化/生理作用
包装
外形
正常兔IgG。1瓶，125 μg纯化兔IgG，溶于125 μL储存液（含0.1%叠氮化钠）。-20°C保存。
ChIP引物，突触蛋白RE1。1瓶，75 μL，人突触蛋白RE1位点特异性引物各5 μM。 -20°C储存。
正向：GGT GCT GAA GCT GGC AGT
反向：TGG GTT TTA GGA CCA GGA TG
制备说明
分析说明
采用2 µg的正常兔IgG或抗REST抗体和 Magna ChIP A试剂盒（货号17-610），对HeLa S3细胞来源的、超声处理的染色质（每次IP需4 X 106细胞）进行染色质免疫沉淀。 使用对照引物通过qPCR验证了REST相关DNA片段的成功免疫沉淀。
其他说明
免责声明
储存分类代码
10 - Combustible liquids
相关内容
Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
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