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Merck
CN

17-622

ChIPAb+ 三甲基-组蛋白H3（Lys27）- ChIP验证的抗体和引物组

from rabbit, purified by using Protein A

别名:

Chip Antibody and primer set, H3K27me3 ChIP, H3K27me3, Histone H3 (tri methyl K27), Histone H3K27me3, Histone H3K27me3 ChIP

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.32
eCl@ss:
32160702

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产品名称

ChIPAb+ 三甲基-组蛋白H3（Lys27）- ChIP验证的抗体和引物组, from rabbit, purified by using Protein A

biological source

rabbit

clone

polyclonal

purified by

using Protein A

species reactivity

human, mouse

manufacturer/tradename

ChIPAb+
Upstate®

technique(s)

ChIP: suitable
western blot: suitable

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

dry ice

Quality Level

100

Gene Information

human ... H3F3B(3021)

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Analysis Note

对照
1小瓶，含有75 μL的5 μM人α卫星特异性对照引物。储存于-20°C。

用于：CTG CAC TAC CTG AAG AGG AC

REV: GAT GGT TCA ACA CTC TTA CA
染色质免疫沉淀：
使用4 μg纯化抗体或正常兔IgG和Magna ChIP® A试剂盒（目录号17-610）对从3x106 NIH3T3 L1细胞制备的超声染色质进行染色质免疫沉淀。使用ChIP引物人α-卫星通过qPCR验证了与三甲基组蛋白H3
（Lys27）相关的DNA片段的成功富集（请参见图）。
关于实验详细信息，请参阅EZ-Magna ChIP A（目录号17-408）或EZ-ChIP（目录号17-371）试剂盒方案。

Application

三甲基组蛋白H3（Lys27）ChIP验证的抗体&引物组，包括ChIP级抗体 & 特异性对照PCR引物用于H3K27me3染色质免疫沉淀。
染色质免疫沉淀：
使用4 μg纯化的抗三甲基组蛋白H3（Lys27）抗体或正常兔IgG和Magna ChIP A试剂盒（目录号17-610）对从2x106 HeLa细胞制备的超声染色质进行染色质免疫沉淀。使用位于人GAPDH启动子侧翼的ChIP引物GAPDH和靶向人MyoD启动子的引物，通过qPCR验证了三甲基组蛋白H3（Lys27）相关DNA片段的成功富集。
关于实验详细信息，请参阅EZ-Magna ChIP A（目录号17-408）或EZChIP（目录号17-371）试剂盒方案。

蛋白质印迹分析：
重组组蛋白H3（泳道2）和HeLa细胞酸提取物（泳道1）通过电泳分离，转移到硝酸纤维素中，用1:5000稀释的抗三甲基组蛋白H3 Lys27（0.4 μg/mL）进行探测。
使用HRP偶联的山羊抗兔二抗和化学发光检测系统观察蛋白质（请参见图）。
研究子类别
组蛋白
研究类别
表观遗传学&核功能

Biochem/physiol Actions

三甲基组蛋白H3 (Lys27)
预计具有广泛的物种交叉反应性。

General description

17 kDa
每批，
每次对全部ChIPAb+抗体的染色质沉淀进行单独验证。每个ChIPAb+抗体组均包含对照引物（通过qPCR对每个批次进行检测），以在基因座特异性环境下对IP结果进行生物学验证。提供了qPCR方案和引物序列，使研究人员能够在染色质环境中使用我们的抗体时验证ChIP方案。
每组还包括阴性对照抗体，以确保ChIP反应的特异性。ChIPAb+三甲基组蛋白H3（Lys27）组包括抗三甲基组蛋白H3（Lys27）抗体，阴性对照抗体（纯化的兔IgG）和qPCR引物，可扩增人α卫星的139 bp区域。三甲基组蛋白H3（Lys27）和阴性对照抗体以可扩展的“每个ChIP”反应大小提供，可用于功能上验证三甲基组蛋白H3（Lys27）相关染色质的沉淀。
组蛋白的甲基化可以发生在两个不同的残基上：精氨酸或赖氨酸。组蛋白甲基化可以与转录激活或抑制相关，具体取决于甲基化残基。组蛋白H3的赖氨酸27可以通过不同的组蛋白甲基转移酶（例如，EZH2或NSD3）单甲基化、二甲基化或三甲基化（组蛋白H3单甲基Lys27、组蛋白H3二甲基Lys27或组蛋白H3三甲基Lys27）。该残基的甲基化主要与转录抑制有关。

Immunogen

KLH缀合合成的2X支肽，包含序列...AR(me3K)SAP...，其中me3K对应于人组蛋白H3残基27处的三甲基赖氨酸。
表位：三甲基Lys27

Other Notes

抗三甲基组蛋白H3（Lys27）多克隆抗体，1小瓶

多克隆对照兔血清，1小瓶

人α-卫星引物组，1小瓶

Packaging

25次测定/试剂盒，约4 μL/染色质免疫沉淀。

Physical form

抗-三甲基组蛋白H3（Lys9）（兔多克隆IgG）。一小瓶含有100 μg蛋白A纯化IgG，溶于100 μL缓冲液中，缓冲液含有0.1 M Tris-甘氨酸（pH 7.4）、0.15 M NaCl和0.05%叠氮化钠。储存于-20°C。

正常兔IgG。一瓶含125 μg纯化兔IgG，溶于125 μL储存缓冲液（含0.1%叠氮化钠）。储存于-20°C。

ChIP引物人α-卫星。一个小瓶含有75 μL 5 μM人α卫星特异性的对照引物。储存于-20°C。
对于：CTG CAC TAC CTG AAG AGG AC
REV: GAT GGT TCA ACA CTC TTA CA

Preparation Note

自发货之日起在-20°C下保存1年

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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