species reactivity (predicted by homology)
all
manufacturer/tradename
Catch And Release, Upstate®
technique(s)
activity assay: suitable (IP-kinase), immunoprecipitation (IP): suitable, western blot: suitable
shipped in
wet ice
Quality Level
General description
特征&优点:
可重现:消除了通常导致减少/丢失样品的抽吸步骤
方便性:旋转柱形式极大地简化了样品处理
可逆:抗原:抗体复合物以天然或变性形式简单洗脱:
用途:
该试剂盒可通过使用旋转柱进行快速且可重现的免疫沉淀(IP)。 该系统比常规IP′具有更高的重现性,后者在不破坏琼脂糖床的情况下洗涤蛋白A/G琼脂糖方面存在问题。 抗体/抗原复合物在Catch and Release中的结合是可逆的,并且免疫复合物的洗脱可以用天然或变性缓冲液进行。 该系统已通过兔,小鼠,绵羊和山羊抗体成功测试。 使用人IgG1-4的IP应该是合适的。 本试剂盒不建议使用鸡抗体或人IgA、IgD、IgE或IgM进行IP。 使用前请阅读随附的产品手册。
可重现:消除了通常导致减少/丢失样品的抽吸步骤
方便性:旋转柱形式极大地简化了样品处理
可逆:抗原:抗体复合物以天然或变性形式简单洗脱:
用途:
该试剂盒可通过使用旋转柱进行快速且可重现的免疫沉淀(IP)。 该系统比常规IP′具有更高的重现性,后者在不破坏琼脂糖床的情况下洗涤蛋白A/G琼脂糖方面存在问题。 抗体/抗原复合物在Catch and Release中的结合是可逆的,并且免疫复合物的洗脱可以用天然或变性缓冲液进行。 该系统已通过兔,小鼠,绵羊和山羊抗体成功测试。 使用人IgG1-4的IP应该是合适的。 本试剂盒不建议使用鸡抗体或人IgA、IgD、IgE或IgM进行IP。 使用前请阅读随附的产品手册。
Application
Catch and Release v2.0不应与含有6-His标签的蛋白质一起使用。
Packaging
50次免疫沉淀
Preparation Note
全部组分应在4°C下储存,但Catch and Release捕获管除外,后者在室温下储存。 组件自装运之日起6个月内保持稳定。
Analysis Note
通过免疫沉淀进行了常规评估。
Other Notes
Catch and Release 清洗缓冲液,10X(目录号20-210)
Catch and Release 非变性洗脱缓冲液,4X(目录号20-209)
Catch and Release 变性洗脱缓冲液,1X(目录号20-284)
20-285
20-216
Catch and Release 捕获管
Catch and Release 非变性洗脱缓冲液,4X(目录号20-209)
Catch and Release 变性洗脱缓冲液,1X(目录号20-284)
20-285
20-216
Catch and Release 捕获管
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
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Addition of human papillomavirus (HPV) E7 CDK2/cyclin A or CDK2/cyclin E, purified from either insect cells or bacteria, dramatically upregulates histone H1 kinase activity. Activation is substrate specific, with a smaller effect noted for retinoblastoma protein (Rb). The CDK2 stimulatory
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Sharma, Sunita, et al.
Neurobiology of Disease, 32, 426-432 (2008)
Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns.
A Brymora et al.
Analytical biochemistry, 295(1), 119-122 (2001-07-31)
E-Y Huang et al.
Cell death & disease, 3, e251-e251 (2012-01-13)
Galectin-1 is a lectin recognized by galactoside-containing glycoproteins, and is involved in cancer progression and metastasis. The role of galectin-1 in radiosensitivity has not previously been investigated. Therefore, this study tests whether galectin-1 is involved in the radiosensitivity mediated by
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Jiang, X., et al
Stroke, 34, 2958-2963 (2003)
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